The method has limits of detection and quantitation of 0.1344 µg mL-1 and 0.4074 µg mL-1 respectively. A linear response was observed over 2.9 -14.5 µg/mL of nifedipine with a correlation coefficient of 0.9985 and the drug combined with CDNBD at a stoichiometric ratio of 2:1. Optimal temperature and time for coupling were selected as 50 oC and 15 minutes. Nifedipine was reduced with Zn/HCl reduction system and then the diazo coupling reaction was carried out with the CDNBD reagent to generate a new azo adduct with optimal wavelength at 470 nm representing a bathochromic shift relative to nifedipine, reduced nifedipine and CDNBD reagent. Judging from the various generic brands currently now available, this research was conceived as a means of developing an alternative cost-effective and readily adaptable method for the assay of nifedipine in tablets and for which official high performance liquid chromatographic technique may not be readily available. ![]() ![]() ![]() A new sensitive colorimetric determination of nifedipine has been developed following azo dye formation with 4-carboxyl-2,6-dinitrobenzenediazonium ion (CDNBD).
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